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Types of Specimen Collection Containers

Types of Specimen Collection Containers

Blood Collection Containers – Common Vacutainer Tubes



ORDER OF DRAW

 

CAP COLOUR


ADDITIVE

 

BLOOD VOLUME

 

SUITABLE FOR / PURPOSE

NO. OF INVERSIONS AFTER COLLECTION

 

1

 

 

Culture

 

10mL

 

Bacteriology and fungal studies

 

8

 

2

 

 

Sodium Citrate

 

2.7mL

Coagulation studies. The ratio of blood to anticoagulant is critical for valid prothrombin time and activated partial thromboplastin time results.

Note: Fill to mark to ensure correct blood to additive ratio.

 

4

3

ACD Tube

6mL

HLA, tissue typing, DNA studies

8

 

4

 

 

Streck (Cell Free DNA)

 

10mL

 

Cell free Plasma DNA

 

10

 

 

 

5

 




 

Clot Activator (Plain)

 

 

Paediatric tube

 

6mL

 




0.5mL

 

Most chemistry, including drug levels and serological tests that require serum. Also for

immunohaematological tests that require clotted blood such as abnormal blood group antibody tests and crossmatching requests.

 

 

 

5

 

6

 

 

SST II

(Gel)

 

5mL

Chemistry, Immunology and Serology studies. All tests requiring serum except those few that need red cells as well (such as abnormal blood group antibody screen, cold agglutinins).

 

5

 

7

 

 

Trace Element Serum

 

6mL

 

Trace Elements/ Toxicology

 

8

 

8

 

 

Lithium Heparin

 

4mL

Tests requiring plasma or whole blood (such as cytogenetics when using blood DNA) and STAT biochemistry tests such as electrolytes, renal screen, ammonia test.

 

8

 

10

 

 

EDTA (GXM)

 

6mL

 

For cross-matching requests only.

 

8

 



11

 

 

 

EDTA

 

Paediatric tube

 

3mL

 

0.5mL

 

Most haematological tests, HbA1C, Cyclosporine, Homocysteine, and molecular genetics tests (when using blood DNA).

 

 

8

 

12

 

 

Trace Element (K2EDTA)

 

6mL

 

Trace Elements/ Toxicology

 

8

 

13

 

 

Potassium Oxalate

 

2mL

 

Chemistry (Glucose)

 

8

Note: Failure to fulfill above requirements may result in specimen rejection.


Other Specimen Collection Containers


TYPE OF CONTAINERS/DESCRIPTION

COMMENTS

Double eSwab (flocked swabs with Amies media)

  1. Pink eSwab - Sample axilla and groin, swirl in Amies media and discard swab
  1. White eSwab - Sample the anterior nares

and place it in the tube of Amies media

For MRSA PCR Screening.

Please refer to the instructions on page 23

CULTURE SWAB with Amies Transport Media

Sterile viscose swab attached to plastic applicator stick with transport media

For bacteriological culture from all sites. It contains a special transport media which will maintain micro-organism viability but does not promote growth of micro-organisms.

Blue-cap swab is suitable for both aerobic &

anaerobic organisms.

DRY SWAB without Transport Media

Sterile cotton swab attached to plastic

applicator stick

For Chlamydia Trachomatis PCR test.

STOOL CONTAINER

Screw cap (brown) plastic container with attached spatula

For collecting stool specimen for routine examination and culture.

URINE CONTAINER, Sterile (UNIVERSAL CONTAINER)

Sterile plastic container with screw cap

For routine urinalysis and urine culture.

Also suitable for collecting body fluids and sputum for routine examination or culture.


For Genetics Department


TYPE OF SUPPLIES

COMMENTS

BOTTLE OF TRANSPORT MEDIUM

For QF-PCR and Karyotyping on Chorionic Villus or Products of Conception

BOTTLE OF TRANSPORT MEDIUM

For Bone Marrow Karyotyping

 

 

MOTTLED BLACK/TAN

Cell-Free DNA

BCT has chemical additives 10mL x 2

For non-invasive fetal aneuploidy screening (NIPS) tests using maternal blood and circulating tumour DNA testing in oncology. Avoid possible backflow from the tube. Tube contents should not touch stopper or the end of the needle during the collection procedure.

Order of Draw: Perform Cell-Free DNA BCT tubes first. After the tube is filled completely, gently invert 8 to 10 times.

PLAIN STERILE UNIVERSAL BOTTLE

For Amniotic Fluid Karyotyping or QF-PCR

SODIUM OR LITHIUM HEPARIN BLOOD TUBE

For Karyotyping and FISH

EDTA BLOOD TUBE

For majority of Molecular Genetics tests


Miscellaneous Supplies


TYPE OF SUPPLIES/DESCRIPTION

COMMENTS

CAPILLARY TUBE (Heparinised)

Self sealing

For neonatal serum bilirubin test. Collect 2 or 3 capillary tubes (heparinised) of blood.

CONTAINER, with Formalin

Clean container (50mL) half-filled with formalin

Supplied for transporting small histopathology specimens.

GLASS SLIDE

Rectangular 3”x1” glass slide with frosted end

Supplied for collecting pap smear and cytology smears only. Please write patient’s name on the frosted end of slide in pencil.

SLIDE HOLDER (Pap Smear Container) Blue or white plastic holder for 2 slide wells. Supplied with 95% alcohol for fixation of smears.

Supplied for holding cytology and pap smear glass slides only.

SPATULA (Scraper)

Wooden stick with paddle

Use to collect material for cytology smears.

SYRINGES and NEEDLES

Disposable syringes & needles

For collecting blood into non-vacutainer tubes, i.e. conventional screw-top containers.

Sizes available for syringes: 20mL, 10mL, 5mL & 2.5mL

Sizes available for needles: 23G 1”, 22G 1½”, 21G

1½”

VACUTAINER NEEDLE

Green 21G vacutainer needle, regular size

Disposable vacutainer needle to be used together with vacutainer needle holder for drawing blood specimen into vacutainer tubes.

DISPOSABLE VACUTAINER NEEDLE HOLDER

White hollow plastic tube

Used to hold vacutainer needle and the vacutainer tube while collecting blood specimen.

OTHER SUPPLIES, include non-vacutainer collection tubes, thin prep vial with cytobrush etc.

Please call 6278 9188 to request for other supplies.